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shrna targeting cbs  (Vector Biolabs)


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    Vector Biolabs shrna targeting cbs
    Shrna Targeting Cbs, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shrna+targeting+cbs/AAV-h-CBS-shRNA/pm38309259-749-39-49
    Average 93 stars, based on 1 article reviews
    shrna targeting cbs - by Bioz Stars, 2026-09
    93/100 stars

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    shRNA:

    Article Title: The live biotherapeutic SYNB1353 decreases plasma methionine via directed degradation in animal models and healthy volunteers.
    Article Snippet: .. The PyMOL Molecular Graphics System, Version 2.5.4 https://pymol.org/ Tableau Tableau by Salesforce www.tableau.com/ Other Innocage cages Innovive Cat# M-BTM-ADE Alpha-Dri Shepherd Specialty Papers N/A PicoLab Rodent Diet LabDiet Cat# 5053 PMI Nutrition International Certified Primate Chow PMI Cat# 5048 Heparin lined tubes Sarstedt Microvette CB 300 Cat# 16.443.100 shRNA targeting CBS packaged in AAV particles Vector Biolabs N/A (Continued on next page) Cell Host & Microbe 32, 382–395.e1–e10, March 13, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Altis TSQ triple quadrupole mass spectrometer Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/mass-spectrometry/liquidchromatography-mass-spectrometry-lcms/lc-ms-systems/triple-quadrupole-lcms/tsq-altis-triple-quadrupole-ms.html Vanquish Flex UHPLC Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/chromatography/liquidchromatography-lc/hplc-uhplc-systems/ vanquish-flex-uhplc-system.html Prominence-i LC-2030C Plus HPLC Shimadzu https://www.ssi.shimadzu.com/products/ liquid-chromatography/hplcuhplc/i-series/ index.html?utm_source=ssiwebsite&utm_medium=hplcindex&utm_campaign=prod-highlight Agilent 1200 HPLC Agilent https://www.agilent.com/en/product/ liquid-chromatography/hplc-systems Proxeon EASY-nLC 1200 Thermo Scientific https://www.thermofisher.com/order/ catalog/product/LC140 Orbitrap Fusion Lumos mass spectrometer Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/mass-spectrometry/liquidchromatography-mass-spectrometry-lcms/lc-ms-systems/orbitrap-lc-ms.html?

    Article Title: The live biotherapeutic SYNB1353 decreases plasma methionine via directed degradation in animal models and healthy volunteers.
    Article Snippet: Eight-week-oldmale C57BL/6J (The Jackson Laboratory, ME)mice (n=9/group) were fasted overnight and received a single oral (PO) dose of d4-methionine (100 or 400 mg/kg). .. Blood was collected at 0, 1, 2, or 4 h post dosing and processed for d4-methionine measurements. e7 Cell Host & Microbe 32, 382–395.e1–e10, March 13, 2024 Mouse model of elevated homocysteine To develop a mousemodel of elevated homocysteine, shRNA targeting CBS packaged in AAV particles were purchased from Vector Biolabs (Malvern, PA) and used to inject 6-week-old C57BL/6J male mice. ..

    Bioprocessing:

    Article Title: The live biotherapeutic SYNB1353 decreases plasma methionine via directed degradation in animal models and healthy volunteers.
    Article Snippet: .. The PyMOL Molecular Graphics System, Version 2.5.4 https://pymol.org/ Tableau Tableau by Salesforce www.tableau.com/ Other Innocage cages Innovive Cat# M-BTM-ADE Alpha-Dri Shepherd Specialty Papers N/A PicoLab Rodent Diet LabDiet Cat# 5053 PMI Nutrition International Certified Primate Chow PMI Cat# 5048 Heparin lined tubes Sarstedt Microvette CB 300 Cat# 16.443.100 shRNA targeting CBS packaged in AAV particles Vector Biolabs N/A (Continued on next page) Cell Host & Microbe 32, 382–395.e1–e10, March 13, 2024 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Altis TSQ triple quadrupole mass spectrometer Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/mass-spectrometry/liquidchromatography-mass-spectrometry-lcms/lc-ms-systems/triple-quadrupole-lcms/tsq-altis-triple-quadrupole-ms.html Vanquish Flex UHPLC Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/chromatography/liquidchromatography-lc/hplc-uhplc-systems/ vanquish-flex-uhplc-system.html Prominence-i LC-2030C Plus HPLC Shimadzu https://www.ssi.shimadzu.com/products/ liquid-chromatography/hplcuhplc/i-series/ index.html?utm_source=ssiwebsite&utm_medium=hplcindex&utm_campaign=prod-highlight Agilent 1200 HPLC Agilent https://www.agilent.com/en/product/ liquid-chromatography/hplc-systems Proxeon EASY-nLC 1200 Thermo Scientific https://www.thermofisher.com/order/ catalog/product/LC140 Orbitrap Fusion Lumos mass spectrometer Thermo Scientific https://www.thermofisher.com/us/en/ home/industrial/mass-spectrometry/liquidchromatography-mass-spectrometry-lcms/lc-ms-systems/orbitrap-lc-ms.html?

    Article Title: The live biotherapeutic SYNB1353 decreases plasma methionine via directed degradation in animal models and healthy volunteers.
    Article Snippet: Eight-week-oldmale C57BL/6J (The Jackson Laboratory, ME)mice (n=9/group) were fasted overnight and received a single oral (PO) dose of d4-methionine (100 or 400 mg/kg). .. Blood was collected at 0, 1, 2, or 4 h post dosing and processed for d4-methionine measurements. e7 Cell Host & Microbe 32, 382–395.e1–e10, March 13, 2024 Mouse model of elevated homocysteine To develop a mousemodel of elevated homocysteine, shRNA targeting CBS packaged in AAV particles were purchased from Vector Biolabs (Malvern, PA) and used to inject 6-week-old C57BL/6J male mice. ..



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    A, PC12 cells were transfected with <t>Scram-shRNA,</t> <t>CBS-shRNA,</t> Vector for 6 h, respectively, and then the cell supernatant was sucked out and replaced with the normal cells medium. After 24 h incubation, the expression of CBS in PC12 cells was detected by Western blot using an anti-CBS antibody. Western blot images show representative results from three independent experiments. In all blots, β-actin was used as a loading control. B, after transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 24 h, and then the content of H 2 S in cell medium was detected by NNDPD method as described in “ ” section. Values are expressed as the mean ± SEM of three independent experiments. * P <0.05, ** P <0.01, vs vehicle group (control); ## P <0.01, vs FA-treated alone group.
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    Image Search Results


    A, PC12 cells were transfected with Scram-shRNA, CBS-shRNA, Vector for 6 h, respectively, and then the cell supernatant was sucked out and replaced with the normal cells medium. After 24 h incubation, the expression of CBS in PC12 cells was detected by Western blot using an anti-CBS antibody. Western blot images show representative results from three independent experiments. In all blots, β-actin was used as a loading control. B, after transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 24 h, and then the content of H 2 S in cell medium was detected by NNDPD method as described in “ ” section. Values are expressed as the mean ± SEM of three independent experiments. * P <0.05, ** P <0.01, vs vehicle group (control); ## P <0.01, vs FA-treated alone group.

    Journal: PLoS ONE

    Article Title: A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide

    doi: 10.1371/journal.pone.0054829

    Figure Lengend Snippet: A, PC12 cells were transfected with Scram-shRNA, CBS-shRNA, Vector for 6 h, respectively, and then the cell supernatant was sucked out and replaced with the normal cells medium. After 24 h incubation, the expression of CBS in PC12 cells was detected by Western blot using an anti-CBS antibody. Western blot images show representative results from three independent experiments. In all blots, β-actin was used as a loading control. B, after transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 24 h, and then the content of H 2 S in cell medium was detected by NNDPD method as described in “ ” section. Values are expressed as the mean ± SEM of three independent experiments. * P <0.05, ** P <0.01, vs vehicle group (control); ## P <0.01, vs FA-treated alone group.

    Article Snippet: The short hairpin RNA (shRNA) targeting rat CBS gene (shRNA CBS) and the scrambled shRNA control (shRNA control) were obtained from OriGene Technology Inc (Rockville, MD, USA).

    Techniques: Transfection, shRNA, Plasmid Preparation, Incubation, Expressing, Western Blot

    After transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 24 h. A, the viability of PC12 cells was determined by CCK-8 assay. B, the apoptosis of PC12 cells was visualized under a fluorescence microscope (10 × objective, BX50-FLA, Olympus) after incubated with 5 mg/L Hoechst 33258 for 30 min. Values are expressed as the mean ± SEM of three independent experiments. ** P <0.01, vs scrambled shRNA group; ## P <0.01, vs FA-treated alone group.

    Journal: PLoS ONE

    Article Title: A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide

    doi: 10.1371/journal.pone.0054829

    Figure Lengend Snippet: After transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 24 h. A, the viability of PC12 cells was determined by CCK-8 assay. B, the apoptosis of PC12 cells was visualized under a fluorescence microscope (10 × objective, BX50-FLA, Olympus) after incubated with 5 mg/L Hoechst 33258 for 30 min. Values are expressed as the mean ± SEM of three independent experiments. ** P <0.01, vs scrambled shRNA group; ## P <0.01, vs FA-treated alone group.

    Article Snippet: The short hairpin RNA (shRNA) targeting rat CBS gene (shRNA CBS) and the scrambled shRNA control (shRNA control) were obtained from OriGene Technology Inc (Rockville, MD, USA).

    Techniques: Transfection, shRNA, CCK-8 Assay, Fluorescence, Microscopy, Incubation

    After transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 9 h and the accumulation of intracellular ROS in PC12 cells were visualized under a fluorescence microscope (10 × objective, BX50-FLA, Olympus) after DCFH-DA staining for 30 min.

    Journal: PLoS ONE

    Article Title: A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide

    doi: 10.1371/journal.pone.0054829

    Figure Lengend Snippet: After transfection with CBS-shRNA for 6 h, PC12 cells were exposed to 120 µmol/L of FA for 9 h and the accumulation of intracellular ROS in PC12 cells were visualized under a fluorescence microscope (10 × objective, BX50-FLA, Olympus) after DCFH-DA staining for 30 min.

    Article Snippet: The short hairpin RNA (shRNA) targeting rat CBS gene (shRNA CBS) and the scrambled shRNA control (shRNA control) were obtained from OriGene Technology Inc (Rockville, MD, USA).

    Techniques: Transfection, shRNA, Fluorescence, Microscopy, Staining